Preparation of Cicer Artienium Amylase Loaded BSA Nanoparticles and Their Bioproteolysis to be Used as Detergent Additive

Cicer aritenium is rich source of amylase and used for extraction of amylase. Amylases are known for their industrial application in food, pharmaceutical, detergent, textile and leather industries. The extracted amylase was encapsulated into biochemically engineered BSA nanoparticles with butanol through glutaraldehyde by desolavtion method. BSA was chosen as a biocompatible matrix used for the binding of amylase where 77.6% of enzyme encapsulation was achieved. This binding was lead to enhancing the thermal stability at 72oC for 6 hours and storage stability at 4oC of bound enzyme for 16 months as compared to free enzyme whose thermal stability at 72oC only for 15 minutes and storage stability only for 24 hours day were found. However, studied thermal stability, storage stability and % of encapsulation for maximal activity for encapsulated enzyme was found to be at remarkably higher as compared to free enzyme which increased the industrial viability of amylase after immobilization. Characterization of prepared nanoparticles was done by Dynamic Light scattering (DLS) and Scattering Light Microscopy (SEM). Observed size of enzyme loaded nanoparticles was observed in the range of 56 nm to 107.4 nm. Bioproteolysis of enzyme loaded BSA nanoparticles was performed by alkaline protease (10 U, 15 U, 20 U, 25 U, 30 U, 35 U, 40 U, 45 U) for sustained and controlled release of encapsulated amylase from prepared nanoparticles. And, further, their application was studied with detergents powder (Aerial, Surf Excel, Wheel and Tide) for washing of tough stained cloths pieces having chosen stains of coffee, tea, pomegranate and turmeric. These strains are found to be difficult to vanish in one simple wash. But, this cost effective bio-active enzyme loaded BSA nanopreaparation were found to be effective in washing with chosen detergents for quick and efficient washing with absolute clear visible results as compared to the results when washing of stained cloths pieces was done with detergents only.