Crystallization of the delta toxin of Staphylococcus aureus

Abstract Delta toxin is a small cytolytic polypeptide produced and secreted by the organism Staphylococcus aureus and belongs to a family of surface-active toxins that exhibit pronounced effects on a wide variety of cellular membranes. Although this class of proteins has been much studied by a wide variety of physical techniques, no consensus has been reached on their mode of action. Therefore, in order to investigate their role in causing membrane damage, a structural analysis of the delta toxin has been initiated. Crystals of this protein have been grown by dialysis against mixtures of 2-methylpentan-2.4-diol and water. These crystals are relatively insensitive to radiation damage and diffract to high resolution. The results of this study should provide a valuable insight into the cytolytic properties of these molecules.