observation of reproductive organs and oocyte^s character of female tree shrews anesthetized.

ObjectiveTo observe the reproductive organs and oocyte's character in the narcosis of female tree shrews. MethodFemale tree shrews were anesthetized by pentobarbital sodium im(1%,10 -3ml/g), and the anesthetic effect at different temperatures(25-28℃,18-20℃) were compared. Results1.The duration of narcosis was 80 min and 130 min at 25-28℃ and 18-20℃ respectively. 2.The uterus of tree shrew is double horns, and ovary is enveloped;3.The oocyte has abundant pigment. Its zona -pellucide and cytoplasm membrane are tougher than goat's. ConclusionIt is well suitable for female tree shrew in dose of 1% pentobarbital sodium (10 -3ml/g) at 25-28℃ because of proper duration of narcosis. The anatomy structure of female reproductive organs and oocyte's character show that embryo transfer and zygote microinjection of tree shrew are similar to mice's.