Bacteriological detection of Salmonella in the presenceof competitive micro-organisms
2001
A fifth bacteriological collaborative study organised by the Community
Reference Laboratory for Salmonella had as participants the National
Reference Laboratories for Salmonella (NRLs-Salmonella) of the EU Member
States. The objectives were: 1) To evaluate the results of the detection
of different contamination levels of Salmonella Enteritidis (100 and 500
cfp) and Salmonella Typhimurium (10 and 100 cfp) in the presence of
competitive micro-organisms among and within the NRLs-Salmonella, and 2)
To evaluate MSRV as selective enrichment medium compared to RV, used as
the selective enrichment medium in the standard method. An adapted ISO
6579 (proposed reference) method was used in combination with MSRV and,
optionally, the laboratory's own routine method for the detection of
Salmonella in chicken faeces. Significantly more positive isolations
were obtained with the STM100 capsules than with the STM10, SE100 and
SE500 capsules. The number of positive isolations from the SE100
capsules was also significantly lower than the number of positive
isolations from the STM10 and SE500 capsules. Significantly more
positive isolations were obtained using MSRV compared to using RV.
Significant differences were also found between laboratories. When
compared with RV, the use of MSRV led to significantly more positive
isolations from the naturally contaminated samples containing S.
Enteritidis.
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