The immunohistochemical localization of signal-transduction pathway components Jak1, Jak2, Jak3, Tyk2 and STAT-1 during early enamel and dentine formation in rat molars.

Abstract This study sought to localize immunohistochemically Janus kinase (Jak) and Tyk isoforms and STAT-1 in association with events involved in early dentine and enamel formation in the rat molar. The Jaks and STATs (signal transducers and activators of transcription) are key signal-transduction pathway components in the cytokine receptor-linked pathway. The histological sections were not demineralized or fixed, providing optimum conditions for immunohistochemical localization. It appears that all of the Jak isoforms and STAT-1 are involved in enamel formation. Jak2 and STAT-1 colocalized in the proximal ends of presecretory and secretory-stage ameloblasts, supporting work by others that growth. hormone receptor is located at that site. The colocalization of Jakl, Jak2 and STAT-1 along the proximal ends of presecretory and secretory ameloblasts suggests that the interferon receptor is up-regulated in these cells as well. Also, colocalization of Jak3 and STAT-1 in the proximal ends of the ameloblasts and the cells of the stratum intermedium predicts the location of the interleukin-7 receptor in those locations. Jakl, Tyk2 and STAT-1, but not Jak2 or Jak3, stain was seen in the odontoblasts.