MINIMUM BIOFILM ERADICATION CONCENTRATION OF Copaifera paupera AGAINST THE BACTERIA THAT CAUSE ORAL INFECTION

Purpose: The goal of this study was to analyze the minimum biofilm eradication concentration (MBEC) of the resin-oil of the plant species C. paupera against bacteria that causes oral pathologies with in-vitro assays models. Methods: The Minimum Biofilm Eradication Concentration activity was determined using the number of viable microorganisms that remain adhered to a microtiter plate after an incubation period. After this the supernatant was aspirated from the plate and the biofilm was exposed to the resin-oil, going back to the incubation. Ending this time, it was homogenized with specific broth, and plated into an agar plate divided in 8 parts. After the incubation period the number of colonies were counted, and the results expressed as log10 in colonyforming units per milliliter (CFU/mL), and shown graphically. We used eleven bacteria, which five were standard strains (ATCC), and six were clinical isolates. Results: The resin-oil showed total antibiofilm activity to five strains, Streptococcus sanguinis – ATCC (100 µg/mL) and clinical isolate (200 µg/mL), Streptococcus salivarius – ATCC (200 µg/mL) and clinical isolate (100 µg/mL) and finally Peptostreptococcus micros – clinical isolate (200 µg/mL). All the others bacteria do not showed sensibility. Conclusion: This work has pointed out that the resin-oil has the effective activity on disinfecting biofilms of the most part of bacteria that cause oral infection. The test showed that not only interspecies but also intraspecies variability were found to influence the eradication efficacy. Therefore, we understood that the resin-oil of C. paupera has a good potential as a natural antibiofilm agent.