Changes in phosphorylation of chicken breast muscle in response to L-histidine introduction under low-NaCl conditions

espanolEn un intento por regular positivamente la calidad de la carne a baja concentracion de NaCl, el presente estudio investigo como influye la introduccion de L-histidina (L-his) en la fosforilacion post-mortem de la proteina del musculo de la pechuga de pollo curada durante 16 horas a 1% de NaCl despues de 90 minutos post-mortem. Los resultados de este procedimiento permitieron verificar la presencia de 633 fosfoproteinas diferentes (﹥1.2 veces) inducidas por la introduccion de L-his durante la salazon; la mayoria de las mismas estaba implicada en la glucolisis, la via de senalizacion del calcio y la contraccion muscular. Ademas, se comprobo que las principales proteinas expresadas diferencialmente (DEPs) asociadas con la contraccion muscular fueron la miosina, la actina, la titina, la troponina C (TnC), la cadena ligera de miosina quinasa (MLCK) y la γ-fosforilasa quinasa (γ-PHK). Al respecto, tras el tratamiento con L-hisse se obtuvo una regulacion ventajosa de la fosforilacion de la fosfoglucomutasa-1, la fructosa-1,6-bisfosfatasa y el componente E1 de la enzima piruvato deshidrogenasa en el metabolismo glucolitico. EnglishIn an attempt to positively regulate meat quality at low NaCl concentration, the influence of L-histidine (L-his) introduction on the postmortem phosphorylation of chicken breast muscle protein that had been cured for 16 h at 1% NaCl was investigated at 90 min postmortem in the present study. The 633 different phosphoproteins (﹥1.2-fold) induced by L-his introduction during salting were identified, and a majority of these proteins were involved in glycolysis, calcium signalling pathway and muscle contraction. Myosin, actin, titin, troponin C (TnC), myosin light-chain kinase (MLCK) and γ-phosphorylase kinase (γ-PHK) were the main differentially expressed proteins (DEPs) that were associated with muscle contraction. An advantageous regulation of phosphoglucomutase-1 phosphorylation, fructose-1,6-bisphosphatase and pyruvate dehydrogenase E1 component was obtained in glycolytic metabolism after treatment with L-his.