수영운동이 비만 zucker rat의 골격근 TNF-α 및 백색지방조직 UCP-2 발현에 미치는 영향

The purpose of this study was to investigate the expression on TNF-α of skeletal muscle and UCP-2 of white adipose tissue with glycometabolism according to 12 weeks of swimming in Zucker rat. Zucker rat were randomly divided into two groups (n=7 in each group): control, swimming group. Animals in swimming group were subjected to swim. Swimming exercise was conducted daily in swimming pool for 30min five days a week for 12 consecutive weeks (with free weight). Rats were sacrificed 24 hrs after the last bout of exercise. Soleus muscle and white adipose tissue were extracted to analyze for TNF-α and UCP-2, respectively. TNF-α and UCP-2 variables were analyzed using the Western Blotting technique. A independent t-test was used to examine the difference between groups. The present results demonstrated that in swimming group, fasting glucose levels significantly decreased in swimming group compared to control (p＜.001). But no significant differences in levels of serum insulin were found between groups. UCP-2 induction levels in white adipose tissue further increased in swimming group compared to control groups (p＜.001). Also swimming exercise significantly decreased expression of TNF-a in soleus muscle of Zucker rats. The present data suggested that low-intensity swimming exercise may improve glycometablism control by up-regulating UCP-2 expression and down-regulation the TNF-α of skeletal muscle in Zucker rats.