Optimization of Culture Conditions for Production of Xylanase from Thermomyces Lanuginosus by Liquid State Fermentation

Xylanase is an important enzyme having various biotechnological applications. The present work focused on optimization of culture conditions for enhanced enzyme production. The maximum production was observed at 40 0 C (15.20±0.20 U/ml) after 96h of incubation (15.20±0.20 U/ml) and at pH 6.5 (15.20±0.20 U/ml. Maximum enzyme production was observed on wheat bran as substrate (15.20±0.20 U/ml) and at 1.5% wheat bran (18.10±0.10 U/ml. Maximum xylanase production was observed on yeast extract (18.10±0.10 U/ml) and at 1.5% level (20.10±0.10 U/ml). All additives and detergents decrease the activity of enzyme. Maximum decrease was observed when tween-80 was added (10.15±0.21U/ml). Ca 2+ and Mn 2+ were found to enhance xylanase production.