Random amplified polymorphic DNA PCR in the microbiology teaching laboratory: Identification of bacterial unknowns*

There continues to be a need for authentic and engaging undergraduate teaching laboratory investigations that reflect modern molecular biological research methods. We adapted random amplified polymorphic DNA (RAPD) analysis for the microbiology laboratory as a practical activity that allowed students to generate and analyze data for the identification of bacterial unknowns. The activity gave students an appreciation of the value of applying a molecular biological method to a discipline-specific question. The abbreviation used is: RAPD, random amplified polymorphic DNA.