Ability of the influenza viruses and their envelope proteins to stimulates endothelial cells apoptosis in vitro

: The ability of the modern epidemic strains of influenza virus type A (subtypes H5N1, H3N2, H1N1pdm) and their surface proteins, hemagglutinin (HA) and neuraminidase to cause the activation of the cellular protein caspase-3 and stimulate the emergence of phosphatidylserine on the membrane of human endothelial cell line EAhy926 has been studied. It was questioned how the viruses and their surface proteins that were studied cause the activation of caspase-3 after 0.5 h of exposure that recorded immunogistotsitohimicheski. The test viruses and neuraminidase (concentration 10 μg/ml) led to the appearance of phosphatidylserine on the cell membrane in a time interval of 2-8 h from the beginning of the treatment, which was recorded by flow cytometry. The death of endothelial cells when exposed to the HA (in a concentration of 50 μg/ml) and in the same time frame was not accompanied by the appearance of phosphatidylserine. The specific feature of apoptotic cell death during the reproduction of the virus are described, as well as the effects of viral proteins.