Microalgae Culture Using the DASGIP ® PBR4 Module for Illumination with a New Brunswick ™ CelliGen ® 310 Stirred-tank Bioreactor

The number of bioprocess applications for microalgae has increased in recent years, particularly in the fi eld of biofuel production. The combination of the New Brunswick CelliGen 310 stirred-tank bioreactor and the DASGIP LED Illumination System creates a bioreactor setup which is capable of supporting high density microalgal growth. Using the stand-alone Eppendorf DASGIP PBR4 Module, LED illumination spectra and intensities can be controlled for optimal support of all types of chlorophylls and carotenoids. For this study in which high density culture of up to 1.5 x 10 7 cells/mL was achieved, the unicellular freshwater alga, Dunaliella tertiolecta, was used.