Effects of FHIT gene transfection into human pancreatic adenocarcinoma cells on tumor necrosis factor sensitivity

Objective In order to determine the effect of wide type FHIT gene on tumor necrosis factor (TNF) sensitivity of human pancreatic adenocarcinoma,wide type FHIT gene was transduced into human pancreatic adenocarcinoma 1990 cells in which FHIT gene was lost totally.Methods By the method of liposome transfection,FHIT gene was transduced into human 1990 cell lines,and the integration and expression of FHIT were detected by RT PCR and western blotting.Then the growth was observed and the cell survival number was acquired after the treatment of TNF.Results Expression of FHIT was detected by RT PCR and western blotting.The growth rate of FHIT transfected 1990 cells and 1990 cells was 32×10 4 cells/well and 78×10 4 cells/well,respectively (P0.01) with their survival rate being 23% and 74% respectively in the presence of TNF (10 3?μg/L),P0.05.Conclusions The expression of FHIT gene can increase the sensitivity of pancreatic adenocarcinoma to TNF.FHIT might be involved in the apoptotic path of TNF.