A Validated Rp-Hplc Method for Estimation of Ciprofloxacin and Tinidazole in Tablet Dosage Form

A simple, specific, precise and accurate Reverse Phase High Performance Liquid Chromatography (RP-HPLC) method was developed and validated for estimation of ciprofloxacin and tinidazole in combined tablet dosage form. The separation was carried out by Inertsil C18(250 x 4.6mm) column with mobile phase phosphate buffers (pH 6.8): acetonitrile (82:18) v/v, at a flow rate of 1.0ml/min. Detection was carried out at 316nm. The retention time of ciprofloxacin and tinidazole was found to be 5.6 and 9.82 min, respectively. The method was validated for linearity, accuracy and precision. Linearity for ciprofloxacin and tinidazole were in the range of 27.5 -82.5μg/ml for ciprofloxacin, and 33μg/ml -66μg/ml for tinidazole and the correlation coefficient was found to be 0.9999 for both the drugs. The mean recovery for ciprofloxacin and tinidazole was found to be 99.7 and 100.4. The developed method could be employed for the routine analysis of ciprofloxacin and tinidazole in combine tablet dosage form.