Cyclic adenosine monophosphate is the second messenger of prostaglandin E2- and vasoactive intestinal polypeptide-stimulated active bicarbonate secretion by guinea-pig duodenum.

Reimer R, Odes HS, Muallem R, Sehwenk M, Beil W, Sewing K-Fr. Cyclic adenosine monophosphate is the second messenger of prostaglandin E2- and vasoactive intestinal polypeptide-stimulated active bicarbonate secretion by guinea-pig duodenum. Scand J Gastroenterol 1994;29:153-159.In a guinea-pig model we determined the intracellular events mediating the response of duodenal epithelial cells to vasoactive intestinal polypeptide (VIP) and prostaglandin (PG) E2. Intravenous administration of VIP (10-9 to 10-7 mol/kg) and PGE2 (10-9 to 10-6 mol/kg) dose-dependently increased duodenal epithelial bicarbonate secretion against an HCO3 concentration gradient, measured by a luminal perfusion technique, in anaesthetized guinea-pigs up to 4.5-fold. This secretion could be mimicked by intraduodenal dibutyryl cyclic adenosine monophosphate (dBcAMP; 10-9 to 10-7 mol/kg). Secretin (10-9 mol/kg) and PGF2α (10-9 to 10-7 mol/kg), both given intravenously, were without effect or considerably less efficient. For VIP and PGE2, s...