IgG, IgA and IgE Reactivities to Sperm Antigens in Infertile Women

Abstract * Corresponding author. e-mail: andrea.brazdova@vscht.cz. Nowadays, World Health Organization (WHO) appoints infertility as a disease. Still more couples are diagnosed with unexplained infertility, it reaches almost 20 % of all cases. Immune system plays an important role: isounization of -immwomen is possible by human sperm cells. For better understanding, it is necessary to detect and characterize antigens recognized by women immune system. Our study is focused on female infertility and is based on the ejaculate sample from four healthy donors. Antigens analyzed by sodium dodecyl sulphate polyacrylamide gel electrophoresis and isoelectric focusing (followed by IgG, IgA and IgE immunoblotting) were detected by forty-five sera from women who were diagnosed with fertility failure. Ten children’s sera were used as negative controls. The immunodominant sperm antigens detected by IgG antibodies were 68 and 123 kDa proteins with pI from 6.60 to 6.62, respectively. Concerning mucosal immunity the most frequently recognized sperm IgA-binding protein reached 130 kDa and pI 6.9, 7.4, 9.5. This study did not prove theassociation of IgE antibodies with unexplained infertility in women.