Regulation of adenosine system at the onset of peritonitis

Background. Adenosine, a potent regulator of inflammation, is produced under stressful conditions due to degradation of ATP/ADP by the ectoenzymes CD39 and CD73. Adenosine is rapidly degraded by adenosine deaminase (ADA) or phosphorylated in the cell by adenosine kinase (AK). From four known receptors to adenosine, A1 (A1R) promotes inflammation by a Gi-coupled receptor. We have previously shown that A1R is up-regulated in the first hours following bacterial inoculation. The aim of the current study is to characterize the inflammatory mediators that regulate adenosine-metabolizing enzymes and A1R at the onset of peritonitis. Methods. Peritonitis was induced in CD1 mice by intraperitoneal injection of Escherichia coli. TNFα and IL-6 levels were determined in peritoneal fluid by enzymelinked immunosorbent assay. Adenosine-metabolizing enzymes and the A1R mRNA or protein levels were analyzed by quantitative PCR or by Western blot analysis, respectively. Results. We found that CD39 and CD73 were up-regulated in response to bacterial stimuli (6-fold the basal levels), while AK and ADA mRNA levels were down-regulated. Cytokine production and leukocyte recruitment were enhanced (2.5-fold) by treatment with an A1R agonist (2chloro-N 6 -cyclopentyladenosine, 0.1mg/kg) and reduced (2.5–3-fold) by the A1R antagonist (8-cyclopentyl-1, 3dipropylxanthine, 1mg/kg). In contrast to lipopolysaccharide, IL-1, TNF and IFNγ, only low IL-6 levels (0.01ng/ml), in the presence of its soluble IL-6R (sIL6R), were found to promote A1R expression on mesothelial cells. In mice, administration of neutralizing antibody to IL6R or soluble gp130-Fc (sgp130-Fc) blocked peritoneal A1R up-regulation following inoculation. Conclusion. Bacterial products induce the production of adenosine by up-regulation of CD39 and CD73. Low IL6–sIL-6R up-regulates the A1R to promote efficient inflammatory response against invading microorganisms.