Co-regulated Intestinal c-kit by Interleukin-17 and Interleukin-10 on Post-infectious Irritable Bowel Syndrome in Mice

Purpose The aim of this study was to investigate the probable immune mechanisms of PI-IBS. Methods C57L/B6 mice, which have been infected by 500 Trichinella larvae for 56 days, were used to PI-IBS model mice, the levels of the intestinal mRNAs and proteins of c-kit, interleukin-10 (IL-10), and IL-17 were measured by RTPCR and western blotting or ELISA. The locations of c-kit were detected using immunohistochemical staining. Results Levels of the c-kit protein and mRNA were up-regulated in the whole PI-IBS mice intestine. Compared to the control bowel segments, IL-17 levels were significantly higher in the duodenum and ileum, and IL-10 levels were lower in jejunum, ileum, and colon. By immunohistochemical staining, the increased signals of the c-kit were mainly detected in the submucosa and myenteron. Conclusions These results suggested that increased levels of the c-kit resulted in the changes of the intestinal motility and visceral sensitivity in PI-IBS mouse model induced by Trichinella Spiralis infection, and which may be coregulated by IL-17 and IL-10 in pathophysiology of PI-IBS.