Biofunctionalized nanodot zirconia-based efficient biosensing platform for noninvasive oral cancer detection

The authors report results of the studies relating to the synthesis of nanodot zirconia that has been utilized for the fabrication of electrochemical biosensing platform for the detection of CYFRA-21-1 biomarker, secreted in saliva samples of oral cancer patients. For the synthesis of nanodot zirconia (ndZrO2), the hydrothermal process was used and further functionalized with 3-aminopropyl triethoxysilane (APTES). Electrophoretic deposition technique was employed for its deposition onto the ITO electrode. The EDC-NHS reaction was used for anti-CYFRA-21-1 immobilization and bovine serum albumin (BSA) was used for blocking of the nonspecific binding sites. The fabricated biosensing platform (BSA/anti-CYFRA-21-1/APTES/ndZrO2/ITO) exhibited a wide linear detection range (0.5–50 ng/mL) with excellent sensitivity (0.53 µA mL/ng cm2).