AN ENZYMATIC METHOD TO OBTAIN A NEW SCAFFOLD FOR ENGINEERING CARTILAGE

The purpose of this study was to achieve a descellularized scaffold from cartilage tissue, which can be used as xenograft for cartilage tissue regeneration. This work presents the results obtained using one method to wash porcine trachea in order to remove cellular material from the extracellular matrix and to avoid the immune reaction using enzymatic detergent and partial enzymatic degradation with Deoxyribonuclease I (DNase-I), Ethylenediaminetetraacetic Acid (EDTA) and Trypsin. This treatment was qualitatively evaluated by Scanning Electron Microscopy (SEM), and HE this procedure has the potential to avoid rejection reactions of the xenograft.