Cordyceps sinensis hirsutella sinensis purine anabolism enzyme, gene thereof, and application thereof

The invention relates to a purine nucleosidase participating in purine-nucleoside-initiated anabolism of purine such as adenine, guanine, hypoxanthine, xanthine, and the like. The purine nucleosidase is obtained from a Bailing production fungus which is cordyceps sinensis hirsutella sinensis. The invention also relates to a purine nucleosidase coding gene, and an application of the purine nucleosidase. The amino acid sequence of the purine nucleosidase is represented as SEQ ID No.1. The nucleotide sequence of the coding gene is represented as SEQ ID No.2. From the respective of the principle, the invention makes detailed researches in the metabolic pathway in purine synthesis of purine nucleoside. The cloned DNA of the nucleotide sequence provided by the invention can be transferred into engineering fungus through methods of transduction, transformation, and conjugation transferring. Through the regulation upon the expression of biosynthesis gene of purine, high-expression is provided for host purine, such that an effective way for increasing purine yield is provided. Therefore, the invention has important application prospect.