Gender-related differences observed among immune cells in synovial fluid in knee osteoarthritis

Summary Objective There is no existing comprehensive report on the cellular composition of synovial fluids (SFs) from knee osteoarthritis (OA). We therefore aimed to characterise the immune cell composition in SFs from knee OA and in subgroups according to gender. Design The immunophenotyping of monocyte/macrophage lineage cells, T and B cells, NK cells, neutrophils, dendritic and mast cells present in SFs from 53 patients (24 males/29 females) with knee OA was performed using 6-colour flow cytometry. Results SFs from patients with OA contained 90% hematopoietic cells. Lymphocytes were the predominant cell population (44.8%) in the SFs of OA patients, with CD4 + T lymphocytes being more prevalent than CD8 + T cells (CD4 + /CD8 + ratio=1.3). Within the monocyte/macrophage lineage gating, monocytes accounted for 33.9%, macrophages 14.8%, myeloid dendritic cells 16.4%. The rest of the hematopoietic cells were comprised of neutrophils (8%), NK cells (3.8%), T regulatory cells (1.2%), plasmacytoid dendritic cells (1.1%), mast cells (0.3%). In OA females, a higher percentage of CD4 + T cells ( P =0.023), macrophages ( P =0.012), and a lower percentage of monocytes ( P =0.008) and CD8 + T cells ( P =0.002) were detected in comparison to OA males. Conclusions Based on the immune cell composition of SFs, data mining analysis revealed distinct phenotypes (monocyte- and lymphocyte-predominant) within each gender group. This first study on the cellular complexity of SFs in knee OA showed marked differences between male and female patients. The findings give a rational starting point for patient stratification according to their phenotypes, as is required for phenotype-specific treatment strategies.