In vitro effects of eltanolone on rat myocardium

Background : Eltanolone is a new short-acting intravenous induction agent. However, its effects on intrinsic myocardial contractility remain unknown. Methods : The effects of eltanolone and its solvent (soya bean emulsion) on the intrinsic contractility of rat left ventricular papillary muscles were investigated in vitro (Krebs-Henseleit solution, 29°C, pH 7.40, Ca 2+ 0.5 mM, stimulation frequency 12 pulses/min). We studied contraction ; relaxation ; contraction-relaxation coupling under high and low loads ; and postrest potentiation. Results : Eltanolone (0.1, 0.3, 1, 3, and 10 μg.ml -1 ) induced no significant inotropic effect, as shown by the lack of changes in maximum unloaded shortening velocity and active isometric force. Eltanolone did not significantly modify the contraction-relaxation coupling under low load, suggesting that it did not modify calcium uptake by the sarcoplasmic reticulum. Eltanolone did not significantly modify the contraction-relaxation coupling under high load, suggesting that it did not modify calcium myofilament sensitivity. Eltanolone decreased the postrest potentiation in a concentration-dependent manner (from 150 ± 14% to 118 ± 9% at 10 μg.ml -1 , P < 0.001), suggesting a decrease in the maximum capacity of calcium release by the sarcoplasmic reticulum, whereas its solvent did not. However, eltanolone did not slow postrest potentiation recovery, as shown by the absence of significant changes in the recovery slope, τ (4.5 ± 1.4 vs. 3.8 ± 1.0 beats ; difference not statistically significant). Conclusions : Eltanolone induced no significant inotropic effect on rat myocardium. It induced a decrease in the calcium release function of the sarcoplasmic reticulum, but this effect was not sufficiently important to modify the inotropic properties.