Gold Nanoparticles Synthesized with a Polyphenols-Rich Extract from Cornelian Cherry (Cornus mas) Fruits

Gold nanoparticles (GNPs) were obtained by green synthesis with an extract from Cornus mas fruits (GNPs-CM), characterized by several methods, and their biologic effects were evaluated on two cell lines: HaCaT, normal keratinocytes, and A431, epidermoid carcinoma. GNPs were spherical with sizes between 2 and 24źnm. Their optical spectra had a dominant plasmonic band centered at 525źnm; zeta potential distribution was narrow, centered at ź19.7źmV, and the mean hydrodynamic diameter was 58źnm. GNPs were visualized in both cell types entering the cells by endocytosis. The amount of gold uptaken by the cells was dose and time dependent. The intracellular concentration of Au ions was higher in HaCaT compared to A431 cells. The toxicity of GNPs-CM was dose dependent being significant only when the highest concentrations were employed. A431 cells were less affected compared to HaCaT cells, but the difference was not statistically significant. ROS production was not significant, except in HaCaT cells at the highest concentration. The comet assay revealed no significant supplementary DNA lesions, while the secretion of inflammatory cytokines was modulated by the presence of GNPs only when the cells were additionally irradiated with UVB. These results recommend GNPs-CM for further testing and possible dermatological applications.