Molecular characterization of HIV1 nef protein

In summary , data presented here demonstrate that nef codes for two proteins, Nef-p27 and Nef-p25, by a mechanism of alternative transnational initiation. Nef-p27 is a myristoylated protein, partially associated with various components of the cytoplasmic particulate fraction. Nef-p25 is a soluble monomeric protein found exclusively in the cytosol. The association of Nef-p27 with the cytoskeletal infrastructure maybe relatd to its biological role in vivo , an area under intense investigation. No G-protein-like activity was detected in purified recombinant Nef coded by nef genes of three different HIV1 isolates.