Blue light irradiation combined with plasma perfusion for acute liver failure in dogs

Objective To establish canine models of acute liver failure and explore if plasma perfusion after blue light irradiation for acute liver failure in dogs can markedly decrease their blood bilirubin.Methods Liver failure was surgically induced in 12 healthy male beagle dogs.The dogs with acute liver failure were divided into group A (n=6),treated with plasma perfusion,and group B (n=6),treated with blue light irradiation combined with plasma perfusion.Treatment effects were evaluated by testing and comparing the plasma alanine aminotransferase (ALT),aspartate aminotransferase (AST),total bilirubin (Tbil),direct bilirubin (Dbil),total protein (TB),globulin (GLB),and albumin (ALB) before and after the treatment.Results ALT,AST,Tbil,and Dbil significantly increased but TB,GLB,and ALB had no obvious change in both groups 14 days after the treatment,without statistical differences between the 2 groups (P ＞ 0.05).ALT (A:119.3 ± 69.8,B:112.9 ± 102.2) and AST (A:103.5 ± 20.9,B:118.0 ± 32.6) decreased in both groups after treatment,with no statistical differences between the 2 groups (P ＞ 0.05).After treatment,Tbil and Dbil significantly decreased in both groups (P ＜ 0.05).Tbil and Dbil (22.1 ± 7.8,11.4 ± 3.1) were significantly lower in group B than in group A (95.6 ± 21.5,48.9 ± 12.3b).TB (A:36.8 ± 4.6,B:39.9 ± 6.9),GLB (A:23.5 ± 2.9,B:23.0 ± 2.6),and ALB(A:25.0 ± 4.0,B:28.8 ± 3.0) significantly decreased in both groups,without statistical differences between the 2 groups (P ＞ 0.05).Conclusion Blue light irradiation combined with plasma perfusion is more effective in decreasing blood bilirubin than conventional plasma perfusion. Key words: Liver failure; Plasma perfusion; Bilirubin; Blue light irradiation