Protective effects of astragaloside IV on hypertrophy induced by angiotensinII in primary cultured cardiomyocytes of neonatal rats

Aim To investigate the protective effects and mechanism of astragaloside Ⅳ on hypertrophy induced by angiotensinⅡ(AngⅡ)in primary cultured cardiomyocytes of neonatal rats.Methods Cardiomyo-cytes were incubated with AngⅡ to establish the hypertrophy model of primary cultured cardiomyocytes in neonatal rats.AST 10 mg·L-1 or 20 mg·L-1 were added with AngⅡ simultaneously to observe its protective effects on cardiomyocytes.The diameter and the total protein content of cardiomyocyte were measured.The intracellular free calcium concentration([Ca2+]i),activity of sarcoplasmic reticulum Ca2+-ATPases(SERCA)and activity of calcineurin(CaN)were determined.Results Compared with control group,diameter and total protein content of cardiomyocyte induced by AngⅡ were increased significantly,which were inhibited by AST 10 mg·L-1 and 20 mg·L-1,respectively(P0.05 or P0.01).[Ca2+]i was decreased(P0.01,respectively),the activity of SERCA was increased(P0.01,respectively)and the activity of CaN was decreased(P0.05 or P0.01)by AST 10 mg·L-1 and 20 mg·L-1,respectively.Conclusion Primary cultured cardiomyocytes of neonatal rats are protected by AST from hypertrophy induced by AngⅡ by decreasing[Ca2+]i,increasing the activity of SERCA and inhibiting the activity of CaN.