Bubble inducing cell lysis in a sessile droplet
2014
Cell lysis is a key sample preparation stage in many biomedical studies as DNA extraction and classification require the use of the nucleic acid and proteins released upon decomposition of a cell membrane. We present an effective method of lysing cells suspended in a microliter droplet placed on a super-hydrophobic surface. When a bubble, injected into the sessile droplet, subsequently ruptures, a rapidly moving fluid jet is formed. In this work, cells that are transported within this fluid jet are captured on a separate hydrophilic substrate and are shown to have been lysed.
Keywords:
- Correction
- Source
- Cite
- Save
- Machine Reading By IdeaReader
28
References
3
Citations
NaN
KQI