ISOLATION, CHARACTERIZATION AND IDENTIFICATION OF HIGH SALINITY TOLERANT, HEAVY METAL CONTAMINANT AND ANTIBIOTICS RESISTANT AMYLOLYTIC-THERMOPHILIC PSEUDOMONAS Sp.

Earth is our home and it is in danger. Pollution is threatening its sustainability and carrying capabilities. Heavy metal pollution can cause toxicity by inhibiting metabolic reactions. The Minimum Inhibitory Concentration (MIC) of the isolated samples against Nickel (Ni 2+ ), Lithium (Li 1+ ), Copper (Cu 2+ ), Manganese (Mn 2+ ), Zinc (Zn +2 ) and Iron (Fe +3 ) was determined in solid media. Multiple metal resistances of the isolate associated with resistance to various antibiotics were also determined. Salt tolerance was determined both in solid and liquid media. Identified halo-tolerant, metal resistant bacteria find use in the bioremediation of heavy metal contaminated sewage and waste water, and also for the digestion of the high salt rich soil grown plants. Enzymes from these microbes can be used for various bioremediation purposes. Though amylases originate from different sources (plants, animals, and microorganisms), the microbial amylases are the most abundantly produced and used in industry, due to their productivity and thermo stability. Microbe identified is Pseudomonas lini and show remarkable heavy metal tolerance, high salinity tolerance, antibiotic resistance and amylolytic activities.