An Experimental Study on Cellular Dermal Matrix as a Transplantation Scaffold for Bone Marrow Stromal Cells in Beagle Dogs

Objective To explore the feasibility of the acellular dermal matrix(ADM) as a transplantation scaffold for bone marrow stromal cells(BMSCs) in Beagle dogs.Methods The induced BMSCs were seeded onto ADM and the biocompatibility of ADM and BMSCs were observed in vitro.The ADM and BMSCs complex were then implanted subcutaneously in nude mice as the experimental group,and the only ADM was also implanted at the other side of the nude mice as the control group.Formed tissues were harvested for gross observation and histological analysis at 4 and 8 weeks after implantation.Results The biocompatibility of ADM and BMSCs was good in vitro.In the experimental group,histological analysis demonstrated obvious degradation of ADM,good growth of BMSCs,extensive formation of new tissue and bone-like tissue at 4 weeks after surgery.At 8 weeks after surgery,the degradation of ADM was even more obvious,and more extensive formation of bone was observed.In the control group,less new tissue was formed and the degradation speed of ADM scaffold materials was similar with the experimental group.Conclusion It is feasible for ADM as a transplantation scaffold for BMSCs in Beagle dogs.