Subcellular localization of ethylenediaminetetraacetate in the proximal tubular cell of the rat kidney

Abstract Ca-EDTA- 14 C was administered to rats at three different dose levels, only one of which was capable of inducing vacuolization in proximal convoluted tubular cells within 24 hr. Differential centrifugation indicated that the percentage uptake with all three doses was similar over a period of 30 min to 24 hr. The behaviour of the particles relative to time as well as on sucrose water density gradients suggested that the EDTA- 14 C eventually shares particle space with lysosomal enzymes.