Double-Layer Culture Technique as a Tool for the Selection of Calluses Resistant to Toxic Material from Plant Pathogenic Fungi

In order to select plant cells and calluses for their resistance to phytotoxic compounds produced by pathogenic fungi, we developed two methods of associated cultures of plant and fungus in the same culture medium: a partitioned culture method in “X segmented” Petri dishes, and an adaptation of the double layer technique classically used in microbiology.