Inhibitory effects of total flavonoids of scutellaria baicalensis georgi on S_(180),Hep-A-22 and Bcap-37 tumor cells

Objective To study the inhibitory effects of total flavonoids of scutellaria baicalensis georgi(TFSB) on S180,Hep-A-22 and Bcap-37 tumor cell proliferation in vitro and on S180,Hep-A-22 in mice bearing tumor in vivo.Methods In vitro,S180,Hep-A-22 and Bcap-37 cells were divided into control group and TFSB groups(12.5,25.0,50.0,100.0 mg·L-1).The inhibitory effects of TFSB on proliferation of S180 and Hep-A-22 were measured by XTT colorimetric assay,and Bcap-37 cells were measured by MTT colorimetric assay.In vivo,the mice bearing tumor were divided into control group,CTX group(30 mg·kg-1),high,middle,low doses TFSB groups(200,100,50 mg·kg-1).After the mice bearing S180 and Hep-A-22 tumor cells were treated with TFSB for 15 d,the tumor weights were measured,the inhibitory rates of S180 and Hep-A-22 were calculated and survival of Hep-A22 was measured after administration of TFSB for 10 d.Results TFSB inhibited the proliferation of S180,Hep-A-22 and Bcap-37 cells,IC50 values were 16.04,17.74 and 9.05 mg·L-1,respectively.The tumor weight of mice bearing S180 and Hep-A-22 cells in TFSB groups(200,100,50mg·kg-1) were lowered than that in control(P0.01 or P0.05).The tumor inhibitory rates were increased in dose-dependent manner.The survival of mice bearing Hep-A-22 in TFSB groups were higher than that in control(P0.01).Conclusion TFSB may possess significantly inhibitory effect on proliferation of S180,Hep-A-22 and Bcap-37 cells in vitro and the growth of tumor in mice bearing S180 and Hep-A-22 in vivo.