Quantitative ELISA-polymerase chain reaction at saturation using homologous internal DNA standards and chemiluminescence revelation

INTRODUCTION Because of its great sensitivity, the polymerase chain reaction (PCR) is the method of choice for analysis of the expression of rare transcripts from a small number of cells [1, 2, 6, 23]. However, because PCR amplification is exponential, precise measurement of relative or absolute target nucleic acid copy numbers in different samples is difficult. Indeed, small variations in the amplification efficiency from [...]