Penicillial Hydroxamate Siderophore, Accelerates Wound Healing in Diabetic rats

Fungal siderophores are less studied group of microorganisms with vast therapeutic potential. A hydroxamate siderophore has been derived and purified from P. chrysogenum NCIM707. This study investigated for wound healing activity of penicillial hydroxamate siderophore, preliminarily it was tested against five human pathogenic bacteria by disc diffusion and MTT assay. Antioxidant activity was determined by DPPH and H2O2 methods. Human pathogenic DNA cleavage was tested by agarose gel electrophoresis. Angiogenesis activity was evaluated with Chorio allantoic membrane (CAM) assay. Delayed in vivo wound healing was assessed with streptozotocin female Wistar-rats and, this study comprises to estimate hydroxyproline and collagen content in wounded tissue, gene expressions and histopathological analysis of wounded tissue. The hydroxamate siderophore (PHS) showed significant antioxidant, antimicrobial, angiogenic and wound healing capacity.