Purifying method of methicillin-resistant staphylococcus aureus MRSA recombinant protein vaccine I1C

The invention discloses a purifying method of a methicillin-resistant staphylococcus aureus MRSA recombinant protein vaccine I1C, wherein the I1C protein is obtained by recombining and fusing active functional fragments of two antigen molecules of IsdB and ClfA of methicillin-resistant staphylococcus aureus and expressing escherichia coli genetically engineered bacteria. The high purity MRSA recombinant dual subunit genetic engineering protein I1C is obtained by technologies on the genetically engineered bacteria such as high pressure breaking, salting out, GST (Glutathione S Transferase) affinity chromatography, PP (Prescission Protease) digestion, adhere chromatography, gel filtration chromatography and the like. The method disclosed by the invention is simple and rapid in purifying process, easiness in application and good in repeatability. The target protein obtained is high in purity. Animal tests verify that the vaccine can effectively stimulate the organism to generate higher humoral immune response and good immunoprotection.