Avian infectious bronchitis virus IBV-K136, monoclonal antibody cell line 3D5 prepared by using avian infectious bronchitis virus IBV-K136, monoclonal antibodies, and applications of avian infectious bronchitis virus IBV-K136 and monoclonal antibodies

The present invention provides avian infectious bronchitis virus IBV-K136, a monoclonal antibody cell line 3D5 prepared by using the avian infectious bronchitis virus IBV-K136, monoclonal antibodies, and applications of the avian infectious bronchitis virus IBV-K136 and the monoclonal antibodies. According to the present invention, the IBV-K136 strain is an endemic nephropathogenic infectious bronchitis virus strain and is obtained through screen, the IBV-K136 strain is adopted as antigen to amplify and then animal immunization is performed, the immune sera and myeloma cells are fused to screen hybridoma cells, the excellent performance avian infectious bronchitis virus monoclonal antibody cell line 3D5 is screened by using the method, and the cell line 3D5 is culture so as to express a lot of monoclonal antibodies; and the monoclonal antibodies prepared by using the process have characteristics of high monoclonal antibody titer, rapid antigen-antibody reaction, and strong specificity, and is especially suitable for further preparation of the avian infectious bronchitis virus antigen diagnosis kit, and the corresponding detection methods can be the double-antibody sandwich ELISA or colloidal gold method, and the like.