Expression, Purification and Characteristic of Thermostable Alkaline Phosphatase (TAPND27)

Through purification and characteristic determination, it was found that TAPND27 is a octamer with 65 ℃ as its optimal reaction temperature. It remains 49.4% enzymatic activity after incubation at 99 ℃ for 30 min while it remains above 70% enzymatic activity with 5% SDS or Tween20 in the reaction system. It is also stable in wide range pH. The results implied that TAPND27 is suitable for the application on the condition of high temperature and concentration of detergent.