Regulating effect on immunologic function and cytokine by PHA compound preparation in chicken

The immune function influence of ND Immune chicken and the mediation role to Th1/Th2 about the preparation to be explored,the compound preparation was composed with PHA and astragalus polysaccharides.This study detemined immune organs index and the concentrations of IFN-γand IL-10 in different chicken spleen lymphocytes broth and in different days Lasota vaccine immune chicken serum before that the compound preparation were gavaged at doses of 0.05,0.2,0.3 g·kg-1.The results showed that the compound preparation could relate cytokines coordinated.The PHA played a major role in regulating IL-10 in Th2 behalf and astragalus polysaccharides played a major role in regulating IFN-γ in Th1 behalf in vitro.In vivo,the compound preparations could significantly improve the thymus and spleen and bursa of Fabricius indexes.The compound preparations had short-time role of promoting IL-10 in Th2 behalf and turning down IFN-γ in Th1 behalf in immune chicken's peripheral blood.The compound preparations to ND immunity chickens have immuno-enhancing which had relevants to Th1/Th2.