Construction of multiphoton laser scanning microscope based on dual-axis acousto-optic deflector

The construction of a multiphoton laser scanning microscope based on a dual-axis acousto-optic deflector (AOD) is described. Without mechanical inertia and physical movement of the scanner, this system has a high random-addressing and data-acquisition rate of 10μspixel. By proper compensation of the dispersion of the AODs, the spatial resolution of the system is 0.5μm for lateral direction and 1.5μm for axial direction. Fluorescence images are acquired to demonstrate these imaging performances.