Study on Genetic Diversity of 7 Populations of Actinidia by Using Chloroplast Genome Fragment

Actinidia arguta is one of the most complex species in the genus of Actinidia with different ploidy. In this paper, we choose seven representative wild populations of A. arguta in four provinces of China. We use 21 pairs of chloroplast genome hypervariable segments to analyse genetic diversity of seven populations. The result shows that seven pairs within twenty one pairs of primer frag- ment can amplify samples successfully. The No. 13 fragments rpl32-trnL had the best resolution ratio. We used the fragment rpl32-trnL to build the phylogenetic tree of seven populations with MP algorithm and NJ method. The experimental results show that two populations from Changbai Mountain are located at the base of the tree. Three populations from the Dabie Mountains are not in the same branch. At last, the average polymorphic locus of 21 fragments is 27.16. This study provided basis for the development and application of chloroplast genome in Actinidia species.