Determination of Glycoalcaloids in Hungarian Potatoes by HPLC

In this work potato genotypes originated from an intensive resistance breeding programme that includes unique combination of resistance to several pathogens and consumer response were analysed for glycoalcaloid content by reversed phase high performance liquid chromatographic method. The glycoalcaloids were concentrated from potato samples by solid-phase extraction with a disposable C18 cartridge column. Nucleosil 100-5 C-18-AB column was used with 50% acetonitrile in phosphate buffer as the mobile phase. The method was validated according to calibration curve, precision and recovery tests, limit of detection and quantitation. The results indicated that as low as 0.27 µg mL−1 can be quantitatively determined by this method with 94–98% and 99% for recovery and precision, respectively. Hungarian potatoes were analysed by the adapted procedure for their glycoalcaloid content. The result showed that the tested Hungarian potato varieties contain glycoalcaloids between 0.09 and 15 mg 100 g−1. The content of glycoalcaloids in the new varieties of potato was lower than the limit of the Official Food Regulations.