Monitoring of Aflatoxin $B_1$ in Livestock Feeds Using ELISA and HPLC

Because of potential health hazards of aflatoxins for humans, the present study was conducted to monitor aflatoxin B 1 (AFB 1 ) in livestock feeds. A total of 249 samples of feeds collected in Korea were analyzed by DC-ELISA for qualitative analysis of AFB 1 . Then, 27 samples that were verified to contain AFB 1 by DC-ELISA were quantitated by HPLC/FLD. HPLC/FLD analysis revealed that only one sample collected from a farm contained 11 ppb of AFB 1 , whereas the other samples collected from feed companies did not contain AFB 1 . The presence of AFB 1 was further confirmed by LC/MS analysis. TLC analysis indicated that the result of the DC-ELISA was most likely due to possible contamination of other mycotoxins rather than AFB 1 . In conclusion, HPLC/FLD analysis following DC-ELISA is necessary for rapid and accurate detection of AFB 1 .