STUDIES ON HETEROLOGOUS EXPRESSION OF Rhizobium meliloti nifA GENE AND OXYGEN SENSITIVITY OF ITS PRODUCT
1991
When the R. meliloti (Rm) nifA'-lacZ fusion-carrying plasmids were introduced into
the strains of Agrobacterium tumefaciens, R. trifolii and R. astragalus, β-galactosidase
activity was demonstrated. However, activity was not induced by microaerobiosis. Further-
more, R. meliloti nifA'-lacZ fusion was also not expressed in the nodule bacteroids of
R. trifolii and R. astragalus. We speculate that some factor(s) important for the induction
of Rm nifA presumed to be the fixLJ regulatory system would not be operative in these
bacteria.
Experiments using R. meliloti nifH'-lacZ/ K. Pneumoniae nifH'-lacZ fusion and the
constitutive Rm nifA system to test the nifA-dependent expression of nifH'-lacZ under
aerobic and microaerobic conditions in E. coli were performed. The inhibition of the Rm
nifA activation of nifH'-lacZ expression in the bacteria grown in the aerobic condition
was shown. Assays on the Rm nifA-m RNA produced by the constitutive Rm nifA in E.
coli under aerobic and microaerobic conditions with the cloned nifA as a probe for dot
blot hybridization showed a marked decrease of Rm nifA mRNA when the bacteria were
grown under aeration.
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