Killer-cell Inmunoglobulin-like Receptor (KIR) genes as a predictor of response to the anti-HER therapy in solid tumors.

e13024Background: KIRs are an important receptor in natural killer (NK) cells; these cells play a role in antibody-dependent cell-mediated cytotoxicity (ADCC) response due to monoclonal antibodies therapy. It is unknown whether the extensive polymorphism KIRs and/or their human leukocyte antigen (HLA) ligands might influence the response to treatment with monoclonal antibodies (MABs). Methods: We select in this study 39 patients treated with MABs, anti-human epidermal growth factor receptor (anti-HER/EGFR: Trastuzumab for advanced breast cancer and Cetuximab for advanced colorectal and advanced head and neck cancer). All the patients had progressed to the MABs therapy and were grouped in two groups taking into account the time to treatment failure (TTF) duration ( or = 10m).KIR genotyping (16 polymorphisms) was performed by PCR sequence-specific primer technique and KIR-ligand typing was performed for HLA-B & -C loci by reverse PCRSSO methodology and for HLA-C alleles by direct sequencing....