EXPRESS: Age-specific reference intervals of serum anti-Müllerian hormone in Chinese girls.

BACKGROUND This study aimed to establish AMH age-specific reference intervals and determine the correlation between the AMH level and age, body mass index (BMI), and levels of follicle-stimulating hormones (FSH) and luteinizing hormone (LH) in healthy Chinese girls. METHODS Serum AMH levels of 1702 healthy girls (0i-12 years), recruited between March 2018 and December 2019, were determined using the Beckman Access 2 automated chemiluminescence immunoassay. Single-year-specific medians of AMH and effects of age, BMI, FSH, and LH on AMH level were analyzed. RESULTS The AMH median level continued increasing from birth, reached its peak at age 9 at 4.45 ng/mL (interquartile rangei¼»IQRi¼½2.58-6.90), and then gradually decreased. At age 12, the median reached 1.98 ng/mL (IQR 1.05~3.46). Age-specific reference intervals for AMH were established in healthy Chinese girls aged 0â12 years. AMH levels showed a moderately positive correlation with age (r = 0.33, P < 0.001). In contrast, FSH (r = â0.29, P < 0.001) levels were weakly negatively correlated with the serum AMH level. CONCLUSION We established single-year-specific reference intervals for AMH in Chinese girls using the Beckman chemiluminescent platform. This reference range can help clinicians accurately understand AMH secretion in healthy girls and promote its clinical use.