Quantitative evaluation of conformational changes in ribonuclease A as a function of pD

The FT-IR spectra (Amide I band) of Ribonuclease A dissolved in D2O were analyzed. The evolution of peculiar substructures as a function of pD was quantitatively evaluated. The structural change observed at pD equals 3 was related to the cleavage of the salt bridges formed by aspartic and glutamic acids.© (1992) COPYRIGHT SPIE--The International Society for Optical Engineering. Downloading of the abstract is permitted for personal use only.