Improved expression of human rotavirus G1VP7 and G3VP7 antigens in the recombinant adenoviruses by codon optimization

Objective To increase the recombinant adenovirus vector mediated human rotavirus G1VP7 and G3VP7 gene expression through coden optimization. Methods We have artificially synthesized two genes of group A human rotavirus that encode G1VP7 and G3VP7 according to the human biased codan. The modified genes were transfected into 293 cells using adenovirus vectors and the gene products,the respective proteins were produced. The expression level of optimized genes and wild type genes was detected by Western Blot. Results A remarkable increase of the expression level of optimized G1VP7 and G3VP7 genes in comparison with the wild type control. Conclusion The coden optimization indeed help increasing the recombinant adenovirns mediated human rotavirus gene expression,which indicated the potential application of such recombinant adenovirases in the development of adenoviral-vectored rotavirus vaccines. Key words: Codon optimization; Recombinant adenovirus; Rotsvirus