Localization of 5-Nucleotidase and Non-specific Alkaline Phosphatase By Starch Gel Electrophoresis

Summary1. By means of starch gel electrophoresis, 2 alkaline phosphatases were demonstrated, one migrating in the region of alpha-globulin and β-lipoprotein and the other with beta-globulin. 2. 5-nucleotidase constituted 60% of the former, but was not present in the latter. 3. Although alkaline phosphatase which migrated with beta-globulin was elevated in both diseases of bone and liver, it is thought to be derived from bone since it had no 5-nucleotidase. 4. Since 5-nucleotidase is not contained in bone and is not elevated in diseases of the bone, it is thought to be derived from the liver.