Tuning the Porosity of Collagen-based Scaffolds for Use as Nerve Regenerative Templates

Regenerative medicine aims at inducing the formation of physiological tissues, in cases where the spontaneous healing response following a severe injury or disease leads to wound closure via contraction and synthesis of scar or fibrotic tissue. The suppression of both wound contraction and scar synthesis, with the simultaneous synthesis of physiological tissues, might be achieved by implanting a porous macromolecular scaffold within the site of injury, able to host cells and guide their behavior toward regeneration. Tubular scaffolds, reconnecting the proximal and distal stumps of a transected peripheral nerve, demonstrated to be able to induce regeneration of the lost nerve trunk. Experimental evidence from independent investigations shows that protein-permeable porous tubes, of different types, perform better as regenerative templates when their pore size is also cell-permeable (>10 μm). Moreover, the regenerative potential of the porous conduit may be further enhanced when inserting a substrate with lo...